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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant job in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two more splice variants had been documented but in depth nucleotide sequence information and facts isn't nonetheless offered.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best final results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...
Antibodies sure to the blocking peptide not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, you can see which staining is distinct.
Focus on Data Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S period boundary and is degraded as cells development by way of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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